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(11)    Patent No :    AP 2709   
(21)    Application No :    AP/P/2006/003856   
(22)    Filing Date :    12.05.2005   
(24)    Date of Grant &    30.07.2013   
           
(45)    Publication :

(30)  Priority Data       
(33) Country    (31) Number    (32) Date
IN    880/DEL/2004    14.05.2004
IN    2103/DEL/2004    26.10.2004

(84)    Designated State

BW    GM  GH   KE   LS   MW  MZ   NA   SL

SD    SZ   T Z   UG   ZM   ZW
(73) Applicant(s)

NATIONAL RESEARCH DEVELOPMENT CORPORATION,

Anusandhan Vikas 20-22, Zamroodpur Community Centre,
Kailash Colony Extension, New Delhi, 110 048,

India
(72) Inventors
SHARMA Rameshwar Dass, India
NICHANI Anil Kumar, India
(74) Representative
COGHLAN, WELSH & GUEST
ZIMBABWE
(51)  International  Classification   A61K 39/018  (2006.01)    C12N 5/06 (2006.01)
(54)    Title
An anti-theileriosis vaccine.
(57)    Abstract
A process for the preparation of anti-theileriosis vaccine comprising the steps of isolating lymphoid cells infected with schizonts of Theileria annulata present

in an infected animal, culturing the infected cells in a growth medium, subjecting said cultured cells to the step of in-vitro propagation by-serial passaging, developing cloned curltures from a single cell by lim iting dilution technique and further passaging these, karyotyping the cells in the medium to

ascertain the dipioid character, and obtaining viable cells with maximum infectivity rates, determining macroschizont nuclear number, subjecting a number of viable cells with dipioid character to the step of cryoprotectant in liquid nitrogen in the presence of a cryoprotectant in the said growth medium to obtain a suspension of viable cells, testing these cultures at different passages and in varying dose levels in groups of calves for ascertaining their pathogencity / antigenicity, determining passage level and dose for the safety and efficacy of candidate vaccine, its safe use in new born calves and pregnant cows, time taken to induce immunity, serological responses and
(56)    Documents Cited :    PRESTON, P.M. et al    WATHANGA, J M et al    DE 2914813 A1

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