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(11)    Patent No :    AP 2703   
(21)    Application No :    AP/P/2009/004830   
(22)    Filing Date :    28.09.2007   
(24)    Date of Grant &    24.07.2013   
           
(45)    Publication :

(30)  Priority Data       
(33) Country    (31) Number    (32) Date
AU    206905393    28.09.2006
(84)    Designated State
BW    GM    GH    KE    LS    MW  MZ   NA   SL
SD    SZ    T Z    UG    ZM    ZW

(73) Applicant(s)

THE MACFARLANE BURNET INSTITUTE FOR MEDICAL

RESEARCH AND PUBLIC HEALTH LIMITED, Alfred Medical
Research and Education Precinct, Commercial Road,

Melbourne, VIC 3001,
Australi
(72) Inventors
ANDERSON David Andrew, Australia
LLOYD Robyn Elizabeth, Australia
CROWE Suzanne Mary, Australia
et al
(74) Representative
FISHER CORMACK & BOTHA

(51)  International  Classification   G01N 33/68 (2006.01)    G01N 33/53 (2006.01)
C07K 14/705 (2006.01)    G01N 33/569 (2006.01)
(54)    Title
A method of diagnosis and kit therefor.

(57)    Abstract

The invention provides kits and methods for detecting or monitoring the number of cells in sample. The cell comprises a cell surface associated protein (CSAP) comprising a cytoplasmic (cytosolic) and an extracellular (ecto) domain. The kit comprises: (i) a chromatographic device; and (ii) a CSAP-binding agent. The method comprises: (i) optionally contacting the sample with an agent capable of lysing or permeabilizing CSAP bearing cells;

(ii) contacting the sample with a CSAP-binding agent that binds to the cytoplasmic domain of the CSAP; and (iii) directly or indirectly evaluating the

level or presence of bound CSAP in the sample.

(56)    Documents Cited : CARELLA A.V.et al.: PAXTON H. et al.: JOHNSON D. et al.: WO 2006/098724 A1

WO

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