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(11)    Patent No :    AP 2580   
(21)    Application No :    AP/P/2008/004476   
(22)    Filing Date :    08.11.2006   
(24)    Date of Grant &    30.01.2013          
(45)    Publication :
(30)  Priority Data       
(33) Country    (31) Number    (32) Date
ZA    2005/09036    08.11.2005
(84)    Designated State
BW    GM    GH    KE    LS    MW  MZ   NA   SL
SD    SZ    T Z    UG    ZM    ZW
(73) Applicant(s)
SOUTH AFRICAN MEDICAL RESEARCH COUNCIL, Francie van Zijl Drive, Parow, 7925, Western Cape,
South Africa
UNIVERSITY OF CAPE TOWN, Lovers Walk, Rondebosch, Cape Town, 7701,
South Africa

(72) Inventors

RYBICKI Edward Peter, South Africa

TANZER Fiona Lesley, South Africa

(74) Representative


(51)  International  Classification   C12N15/85 (2006.01)    A61K39/21 (2006.01)

(54)    Title

Expression system incorporating a capsid promoter sequence as an enhancer

(57)    Abstract

The invention provides a method for enhancing expression of a transgene in a

host cell, which includes the steps of in serting a capsid promoter element (Pcap), or a sequence comprising the reverse complement of the capsid promoter element (PcapR), into a mammalian expression cassette upstream (5') of a cytomegalovirus immediate/early enhancer/ promoter region (Pcmv); inserting the transgene into the expression cassette downstream (3') of the cytomegalovirus immediate/early enhancer/ promoter region; inserting a vector containing the expression cassette into the host organism; and causing expression of the transgene. The capsid promoter element (Pcap) is typically from a circovirus, parvovirus or anellovirus. The transgene is typically expressed at a higher level than when expressed by a vector containing the expression cassette without the transcriptional control element. An expression cassette, vector, DNA vaccine, pharmaceutical composition and method of

(56)    Documents Cited :    WO 01/61024 A    WO 2007/054788    BURGERS W A et al:    WO 99/45956 A



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