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(11)    Patent No :    AP 1881   
(21)    Application No :    AP/P/2005/003215   
(22)    Filing Date :    03.07.2003   
(24)    Date of Grant &    21.08.2008               
(45)    Publication :
(30)  Priority Data       
(33) Country    (31) Number    (32) Date
EP    02077697.7    04.07.2002

(84)    Designated State

BW    GM    GH    KE    LS    MW  MZ   NA   SL
SD    SZ    T Z    UG    ZM    ZW
 
(73) Applicant(s)

PRIMAGEN HOLDING B. V., Meibergdreef 59, NL-1105 BA

Amsterdam,
The Netherlands

(72) Inventors

DE BAAR Marinus Petrus, The Netherlands

DE ROOIJ Esther Regina, The Netherlands

(74) Representative

HONEY & BLANCKENBERG

(51)  International  Classification   C12M 1/00 (2006.01)    C12N 15/09 (2006.01)
G01N 33/53 (2006.01)   

(54)    Title

Quantification of nucleic acid administered to a solid carrier.

(57)    Abstract

The invention provides a method for detecting a nucleic acid of interest in at least one sample, comprising administering said sample to a solid carrier capable of at least in part absorbing said sample, drying said carrier, providing

at least a representative part of said carrier to a nucleic acid isolation solution so that a representative amount of said nucleic acid is extracted from said carrier, and detecting said representative amount of said nucleic acid. With a method of the invention a sample, such as a body fluid sample, is stabilized in such a way that it can be shipped from the site of taking (for instance local hospital or lab in a less-developed country) and be sent to a service testing laboratory elsewhere in the world by normal logistics means. Preferably at least 100 µl, more preferably at least 250 µl of sample is administered to said carrier, in order to detect low titers of nucle ic acid of interest. A kit of parts for detecting, identifying and/or quantifying a nucleic acid of interest in a sample, comprising a solid carrier capable of at least in part absorbing said


(56)    Documents Cited :    US 5 807 527 A   WO 98 23778 A

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