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(11)    Patent No :    AP 1993   
(21)    Application No :    AP/P/2006/003577   
(22)    Filing Date :    15.09.2004   
(24)    Date of Grant &    09.04.2009   
(45)    Publication :

(30)  Priority Data       
(33) Country    (31) Number    (32) Date
FI    20031319    15.09.2003

(84)    Designated State

BW    GM    GH    KE    LS    MW  MZ   NA   SL
SD    SZ    T Z    UG    ZM    Z

(73) Applicant(s)

FIT BIOTECH OYJ PLC, 33520 Tampere, Biokatu 8,


(72) Inventors

TENSON Tanel, Estonia

LAHT Silja, Estonia

ADOJAAN Maarja, Estonia

et al

(74) Representative



(51)  International  Classification   C12N 9/90 (2006.01)    C12N 15/61 (2006.01)
C12N 9/90 (2006.01)   

(54)    Title

Selection system containing non-antibiotic resistance selection marker

(57)    Abstract

A selection system free of antibiotic resistance genes, which is based on the use of an araD gene as a selection marker carried on a vector which is inserted in a bacterial strain deficient of the araD gene. The araD gene from E. coli encodes the L-ribulose-5-phosphate-4-epimerase. A method of selecting the cells transformed with a plasmid, which contains the araD gene. The non-antibiotic selection marker makes the system suitable for producing

therapeutics. The araD gene is not essential for growth of the host but manipulation of it affects the growth under certain selective conditions.

Deletion of araD leads to accumulation of substance which is toxic to the host but not to humans. The araD gene is relatively small and therefore a small

plasmid may be constructed, which requires less energy for replication, and leads to increased growth rate and yield.

(56)    Documents Cited :    Protein Science, vol. 4, 1995, Arnold Anderson et al   WO 02 090 558 A   Carcinogenesis,



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