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(11)    Patent No :    AP 1980    (73) Applicant(s)       
(21)    Application No :    AP/P/2001/002065    SOUTH AFRICAN SUGAR ASSOCIATION, Mount       
            Edgecombe, Kwazulu Natal,       
(22)    Filing Date :    02.02.2001    South Africa       
(24)    Date of Grant &    23.03.2009           
(45)    Publication :

(30)  Priority Data    (72) Inventors

    (33) Country    (31) Number    (32) Date    BOTHA Frederik Coenraad, South Africa   
    ZA    2000/0574    08.02.2000    GROENEWALD Jan Hendrik, South Africa   
                (74) Representative   
(84)    Designated State           
    KE   MW  MZ    SZ   TZ   UG    ZM   ZW    FISHER CORMACK & BOTHA   
(51)    International    Classification    A01H 1/00 (2006.01)   

(54)    Title

The regulation and manipulation of sucrose content in sugarcane.

(57)    Abstract

This invention relates to the regulation and manipulation of sucrose content in

a sugar-storing plant, such as sugarcane, by regulating the activity of the PFP enzyme in the plant. It has been found that the down regulation of the PFP enzyme by decreasing the concentration of one of subunits, namely the ?-subunit, of the enzyme increases the sucrose content of the plant. In a preferred embodiment of the invention, the activity of the PFP enzyme is down regulated by the introduction of an untranslatable form, or an antisense

(56)    Documents Cited :    EP 0 628 636 A   US 5 387 756 A   Whittaker A. et al; physiologia Plantarum, Vol. 107,



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