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(11) Patent Number: KE 299 (45) Date of grant: 09/02/2009
I.nya Industrial Properly Institute.
(19) Kenya Industrial Property Institute.
(12) PATENT
(51) Int.C1.C: 0 7D 231//54
(21)Application    KE/P! 2006/ 000396    (84) WO No. WO 2005/121099 Al
Number:    22/12/2005
(22) Filing Date:    08/09/2006
(31)Priority Number: 04/05,055    (32) Date: 11/05/2004 (33) Country: FR
(73) Owner(s): SANOFI-AVENTIS of 174, avenue de France, F-75013 Paris., France
(72) Inventor(s)    DUBOIS, Laurent; MALOIZEL, Christian; SEVRIN,
Mireille and EVANNO, Yannick
(74)Agent/address for correspondence: Kaplan & Stratton Advocates, P.O. Box 40111-00100,
Nairobi
(54) Title:    2H OR 3H-BENZO[E]INDAZOL-I-YLE CARBAMATE DERIVATIVES, THE
PREPARATION AND THERAPEUTIC USE THEREOF.
(57) Abstract: The invention relates to compounds of formula (1), wherein W is an oxygen or sulphur atomm, each X, X2 X3 and X4 independently represents a hydrogen or halogen atom or a cyano, C,-C6-alkyl, C-C6-fluoralkyl, C-C6-alkoxy, C,-C6- fluoroalkoxy group, Y is in position (N2) or (N3); when Y is in the (N2) position, Y represents a C,-C6-alkyl, C,-C6-fluoralkyl, aryl or heteroaryl group; when Y is in the (N3) position. Y represents an aryl or heteroaryl group; the ryl and heteroaryl groups are possibly substituted by one or several atoms or groups selected from halogen atoms. C,-C6-alkoxy, C,-C6-alkylthio, C,-C6- alkyl-S(0)-,C,-C6-alkyl-S(0)2-,C,- C6fluoralkyl groups; a C4-05 position bond is double or single; each R1 et R2 independently represents an aryl, benzl or C,-C6-alkyl group or R1 et R2 form, with a nitrogen atom carrier thereof, a heterocycle possibly substituted by one or several Cl-C6alkyl or benzyl groups in the form of a base, an acid addition salt, a hydrate or a solvate. A method for preparation and therapeutic use is also disclosed.
 
1
2H- or 3H-benzo[e]indazol-1-y1 carbamate derivatives,
the preparation and therapeutic use therof
A subject-matter of the invention is 2H- or
5 314-benzo[e]indazol-1-y1 carbamate derivative compounds which exhibit an in vitro and in vivo affinity for peripheral-type benzodiazepine receptors (PBR or p sites).
A first subject-matter of the invention is 10 the compounds corresponding to the general formula (I) below.
Another subject-matter of the invention is processes for the preparation of the compounds of general formula (I).
15    Another subject-matter of the invention is
the uses of the compounds of general formula (I), in particular in medicaments or in pharmaceutical compositions.
The compounds of the invention correspond to 20 the general formula (I):
 
 
2
in which
W represents an oxygen or sulphur atom;
X1, X2, X3 and X4 each represent, independently of one another, a hydrogen or halogen atom or a cyano,
5 Cl-C6-alkyl, Cl-C6-fluoroalkyl, Cl-C6-alkoxy or Cl-C6-fluoroalkoxy group;
Y is in the (N2) or (N3) position;
when Y is in the (N2) position, Y represents a Cl-C6-alkyl, C1-C6-fluoroalkyl, aryl or heteroaryl group;
10 when Y is in the (N3) position, Y represents an aryl or heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from halogen atoms or Cl-C6-alkyl, Cl-C6-alkoxY,
15 Cl-C6-alkylthio, C1-C6-alkyl-S(0)-, C1-C6-alkyl-S(0)2- or Cl-C6-fluoroalkyl groups;
the bond in the C4-05 position is a double or single bond;
R1 and R2 each represent, independently of one another,
20 an aryl, benzyl or C1-C6-alkyl group; or else R1 and R2 form, with the nitrogen atom which carries them, a heterocycle optionally substituted by one or more C1-C6-alkyl or benzyl groups.
In the context of the present invention:
25 - Ct-CZ, where t and z can take the values from 1 to 6, is understood to mean a carbon chain which can have from t to z carbon atoms, for example C1..3, a carbon
 
3
chain which can have from 1 to 3 carbon atoms;
-    an alkyl is understood to mean a saturated, linear or branched, aliphatic group. Mention may be made, by way of examples, of the methyl, ethyl, propyl,
5 isopropyl, butyl, isobutyl, tert-butyl or pentyl groups, and the like;
-    a fluoroalkyl is understood to mean an alkyl group, one or more hydrogen atoms of which have been substituted by a fluorine atom;
    10 -    an alkoxy is understood to mean an -0-alkyl
radical where the alkyl group is as defined above;
-    a fluoroalkoxy is understood to mean an alkoxy group, one or more hydrogen atoms of which have been substituted by a fluorine atom;
    15 -    an alkylthio is understood to mean an -S-alkyl
radical where the alkyl group is as defined above;
-    a heterocycle is understood to mean a 4- to 7-membered cyclic group comprising a nitrogen atom and optionally another heteroatom, such as nitrogen, oxygen
20 or sulphur. Mention may be made, as examples of heterocycles, of the azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, azepinyl, piperazinyl or homopiperazinyl groups;
-    an aryl is understood to mean an aromatic cyclic
25 group comprising between 6 and 10 carbon atoms. Mention may be made, as examples of aryl groups, of the phenyl or naphthyl groups;
 
4
a heteroaryl is understood to mean a 5- or 6-membered aromatic cyclic group comprising 1 or 2 heteroatoms, such as nitrogen, oxygen or sulphur. Mention may be made, as examples of heteroaryl groups,
5 of the pyridyl, thienyl, furyl, pyrimidinyl, pyrazinyl or pyridazinyl groups;
a member is understood to mean, in a cyclic group,
an atom connected to the two adjacent atoms of the ring;
10 -    a halogen atom is understood to mean a fluorine, a
chlorine, a bromine or an iodine.
The compounds of general formula (I) can comprise one or more asymmetric carbons. They can exist in the form of enantiomers or of diastereoisomers.
15 These enantiomers and diastereoisomers, and their mixtures, including the racemic mixtures, form part of the invention.
The compounds of formula (I) can exist in the
form of bases or of addition salts with acids. Such 20 addition salts form part of the invention.
These salts are advantageously prepared with pharmaceutically acceptable acids but the salts of other acids, for example of use in the purification or
the isolation of the compounds of formula (I), also
25 form part of the invention.
The compounds of general formula (I) can exist in the form of hydrates or of solvates, namely in
 
5
the form of combinations or associations with one or more molecules of water or with a solvent. Such hydrates and solvates also form part of the invention.
Among the compounds of formula (I) which are
5 subject-matters of the invention, a first subgroup of
compounds is composed of the compounds for which:
W represents an oxygen or sulphur atom; and/or X1, X2 and X3 each represent, independently of one another, a hydrogen atom, a halogen atom, more
10 particularly a fluorine, chlorine or bromine atom, a cyano group, a C1-C6-alkyl group, more particularly a methyl group, or a C1-C6-alkoxy group, more particularly a methoxy group; and/or
X4 represents a hydrogen atom; and/or
15 Y is in the (N2) or (N3) position;
when Y is in the (N2) position, Y represents a C1-C6-alkyl group, more particularly a methyl or ethyl group, a C1-C6-fluoroalkyl group, more particularly a trifluoroethyl group, an aryl group, more particularly
20 a phenyl group, or a heteroaryl group, more
particularly a pyridyl or pyrazinyl group;
when Y is in the (N3) position, Y represents an aryl group, more particularly a phenyl group, or a
heteroaryl group, more particularly a pyridyl or 25 pyrimidinyl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups, more
 
6
particularly by one or two atoms or groups, chosen from halogen atoms, more particularly fluorine or chlorine
atoms, C1-C6-alkyl groups, more particularly methyl groups, and C1-C6-alkoxy groups, more particularly
5 methoxy groups; and/or
the bond in the C4-05 position is a double or single bond; and/or
R1 and R2 each represent, independently of one another, an aryl group, more particularly a phenyl group, or a
10 Cl-C6-alkyl group, more particularly a methyl, ethyl, n-propyl, t-butyl or isopropyl group; or else R1 and R2 form, with the nitrogen atom which carries them, a heterocycle, more particularly pyrrolidinyl, piperidinyl, morpholinyl or piperazinyl, optionally
15 substituted by one or two C1-C6-alkyl groups, more particularly methyl groups.
Among the compounds of formula (I) which are subject-matters of the invention, a second subgroup of compounds is composed of the compounds for which:
20 W represents an oxygen or sulphur atom; and/or X1, X2 and X3 each represent, independently of one another, a hydrogen atom, a halogen atom, more
particularly a fluorine, chlorine or bromine atom, a C1-C6-alkyl group, more particularly a methyl group, or
25 a C1-C6-alkoxy group, more particularly a methoxy group; and/or
X4 represents a hydrogen atom; and/or
 
7
Y is in the (N2) or (N3) position and represents an aryl group, more particularly a phenyl group, or a heteroaryl group, more particularly a pyridyl, pyrazinyl or pyrimidinyl group;
5 the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups, more particularly by one or two atoms or groups, chosen from halogen atoms, more particularly fluorine or chlorine atoms, C1-C6-alkyl groups, more particularly methyl
10 groups, and C1-C6-alkoxy groups, more particularly methoxy groups; and/or
the bond in the C4-05 position is a double or single bond; and/or
R1 and R2 each represent, independently of one another,
15 an aryl group, more particularly a phenyl group, or a C1-C6-alkyl group, more particularly a methyl, ethyl, n-propyl, t-butyl or isopropyl group; or else R1 and R2 form, with the nitrogen atom which carries them, a heterocycle, more particularly pyrrolidinyl,
20 piperidinyl, morpholinyl or piperazinyl, optionally substituted by one or two C1-C6-alkyl groups, more particularly methyl groups.
Among the compounds of formula (I) which are subject-matters of the invention, a third subgroup of
25 compounds is composed of the compounds for which: W represents an oxygen or sulphur atom; and/or X1, X2 and X3 each represent, independently of one
 
8
another, a hydrogen atom, a halogen atom, more particularly a fluorine, chlorine or bromine atom, a Cl-C6-alkyl group, more particularly a methyl group, or a C1-C6-alkoxy group, more particularly a methoxy group;
5 and/or
X4 represents a hydrogen atom; and/or
Y is in the (N3) position and represents an aryl group, more particularly a phenyl group, or a heteroaryl group, more particularly a pyridyl or pyrimidinyl
10 group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups, more particularly by one or two atoms or groups, chosen from halogen atoms, more particularly fluorine or chlorine
15 atoms, C1-C6-alkyl groups, more particularly methyl groups, and C1-C6-alkoxy groups, more particularly methoxy groups; and/or
the bond in the C4-05 position is a double or single bond; and/or
20 R1 and R2 each represent, independently of one another, an aryl group, more particularly a phenyl group, or a C1-C6-alkyl group, more particularly a methyl, ethyl, t-butyl or isopropyl group; or else R1 and R2 form, with the nitrogen atom which carries them, a heterocycle,
25 more particularly piperidinyl, optionally substituted by one or two C1-C6-alkyl groups, more particularly methyl groups.
 
9
The compounds of general formula (I) can be prepared by the processes illustrated in the following schemes.
According to a first preparation route
5 (Scheme 1), a compound of general formula (II), in which X1, X2, X3 and X4 are as defined in the general formula (I), is reacted with methyl carbonate in the presence of a catalytic amount of a base, such as sodium methoxide or sodium hydride, to obtain the
10 ketoester of general formula (III). The condensation of the ketoester (III) with hydrazine, for example in a polar solvent, such as DMF or acetic acid, makes it possible to isolate the pyrazole of general formula (IV). The latter is subsequently N-substituted
15 nonselectively by the action of an aryl or heteroaryl halide of general formula Y-hal, in which Y is as defined in the general formula (I) and hal is a halogen atom, such as an iodine or a bromine, in the presence of a base, such as potassium or caesium carbonate, or
20 of potassium triphosphate, of a catalytic amount of a
copper salt and of a diamine (S.L. Buchwald, J. Am. Chem. Soc., 2001, 123, 7727).
The resulting mixture composed of the positional isomers of general formulae (Va) and (Vb),
25 in which the Y group is respectively in the 2-position and in the 3-position of the pyrazole ring, is subsequently derivatized by the action of a carbamoyl
 
10
chloride derivative of general formula C1C(W)NR1R2, in which W, R1 and R2 are as defined in the general formula (I), in the presence of a base, such as potassium carbonate, sodium hydride or triethylamine, to obtain
5 the carbamates of general formulae (Ia) and (Ib), which are separated, at this stage, by methods known to a person skilled in the art, such as chromatography on a silica column.
 
11
Scheme 1
Y—hal
 
12
Alternatively, a second preparation route makes it possible to prepare the compounds of general formula (Ia) (Scheme 2).
Scheme 2
It consists in condensing the ketoester of general formula (III), as defined above, with a hydrazine of general formula Y-NH-NH2, in which Y is as
10 defined in the general formula (I), for example in a
 
14
commercially available or are described in the literature or else can be prepared according to methods which are described therein or which are known to a person skilled in the art.
5    The compounds of general formula (II) can be
obtained from commercial sources or can be prepared using methods described in the literature (Sims, J.J. et al., Tetrahedron Lett., 1971, 951).
Another subject-matter of the invention,
10 according to another of its aspects, is the compounds of formulae (Va) and (Vb). These compounds may be of use as intermediates in the synthesis of the compounds of formula (I).
The chemical structures and the physical
15 properties of a few compounds of general formulae (Va) and (Vb) of the invention are illustrated in the following Table 1. The melting points of the products are given in the "M.p." column.
Table 1
20
 
 
15
No.    X1    X2    X3    Y4    Y    M.p. (°C)
Va.1    H    H    H    H    2-(4-methylphenyl)    221-222
Vb.1    H    Me    H    H    3-(pyrid-411)    315-316
Va.2    H    F    H    H    2-(4-fluorophenyl)    220-221
Vb.2    H    CI    H    H    3-ipyrid410    336 - 342
Va.3    H    CI    H    H    2-(pyric1.411)    190-216

The preparation of some compounds in accordance with the invention is described in the following examples. These examples are not limiting and
5 only illustrate the present invention. The numbers of the compounds exemplified refer to those given in Tables 1 and 2. The elemental microanalyses, the LC-MS
(liquid chromatography coupled to mass spectrometry) analyses and the IR and NMR spectra confirm the
10 structures of the compounds obtained.
Example 1 (Compound No. 1) 7-Fluoro-2-(4-fluoropheny1)-4,5-dihydro-214- benzo[e]indazol-1-yl N,N-diethylcarbamate
15    1.1 Methyl 6-fluoro-2-hydroxy-3,4-dihydro-l-
naphthoate
12.66 g (316 mmol) of 60% sodium hydride in oil, 900 ml of toluene and 17.69 ml (210 mmol) of dimethyl carbonate are introduced into a 2 1 reactor.
20 The reaction mixture is stirred at reflux for 1 h. A solution of 19 g (115 mmol) of 6-fluoro-3,4-dihydro-11I¬naphthalen-2-one in 350 ml of toluene is subsequently
 
16
added. The reaction mixture is heated at reflux for 24 h. The reaction mixture is subsequently cooled to
0°C and then acidified by addition of 114 ml of acetic acid. 114 ml of water are added and the separated
5 organic phase is separated by settling and washed with two times 150 ml of water and then with 100 ml of a saturated aqueous sodium chloride solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced pressure to result in
10 26.1 g of a product which is used as is in the following stage.
1.2 7-Fluoro-2-(4-fluoropheny1)-1-hydroxy¬4,5-dihydro-2H-benzo[e]indazole (Va.2)
2 g (9 mmol) of the product obtained in Stage
15 1.1 and 2.73 g (16.8 mmol) of 4-fluorophenylhydrazine hydrochloride are introduced into a 100 ml reactor. The mixture is dissolved in 100 ml of acetic acid and heated at reflux for 4 h. The reaction mixture is subsequently cooled and then concentrated under reduced
20 pressure. The residue is taken up in 150 ml of ethyl acetate and 100 ml of water. The organic phase is separated by settling and washed twice with 100 ml of water and then once with 100 ml of a saturated aqueous sodium chloride solution. The organic phase is
25 subsequently dried over magnesium sulphate and then concentrated under reduced pressure to result in 3.5 g of the expected compound.
 
17
Melting point: 220-221°C
1H NMR (d6-DMS0): 8 (ppm): 2.72 (dxd, 2H), 2.95 (dxd, 2H), 7.01 (m, 2H), 7.3 (m, 2H), 7.75 (m, 3H).
1.3 7-Fluoro-2-(4-fluoropheny1)-4,5-dihydro¬5 21I-benzo[e]indazol-1-y1 N,N-diethylcarbamate
(Compound No. 1)
3.5 g (9 mmol) of the product obtained in Stage 1.2, 3.48 g (25 mmol) of potassium carbonate and 2.66 ml (21 mmol) of N,N-diethylcarbamoyl chloride are
10 introduced into a 500 ml reactor. The reaction mixture is heated at reflux for 24 h and is then concentrated under reduced pressure. The resulting product is taken
up in 100 ml of ethyl acetate. The organic phase is washed twice with 100 ml of water and then once with
15 100 ml of a saturated aqueous sodium chloride solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced pressure to result in 5.96 g of crude product. The mixture is purified by chromatography on a silica column, elution
20 being carried out with a mixture of cyclohexane and ethyl acetate. 2.2 g of the expected product are thus isolated and recrystallized from isopropanol to produce 1.5 g (3.77 mmol) of the final product.
Melting point: 141-142°C
25 111 NMR (CDC13) : 6 (ppm) : 1.95 (t, 3H) , 2.6 (t, 3H) , 2.97 (t, 2H), 3.98 (t, 2H), 3.4 (q, 2H), 3.52 (q, 2H), 7.00 (m, 2H) , 7.2 (m, 2H) , 7.32 (m, 1H) , 7.56 (m, 2H).
 
18
Example 2 (Compound No. 2)
7-Fluoro-2-(4-fluoropheny1)-21I-benzo[e]indazol-1-y1 N,N-diethylcarbamate
5    A solution of 0.7 g (1.76 mmol) of
7-fluoro-2-(4-fluoropheny1)-4,5-dihydro-21I-benzo[e]indazol-1-y1 N,N-diethylcarbamate, obtained in Stage 1.3 of Example 1, and of 1.2 g (5.2 mmol) of 2,3- dichloro-5,6-dicyano-1,4-benzoquinone in 25 ml of
10 toluene is stirred at reflux for 2 h and then cooled.
The mixture is poured onto 100 ml of ethyl acetate. This organic phase is washed with two times 100 ml of a saturated aqueous sodium hydrogencarbonate solution, with 100 ml of water and then with 100 ml of a
15 saturated aqueous sodium chloride solution. The organic phase is separated by settling, then dried over magnesium sulphate and concentrated under reduced
pressure. After purification by chromatography on a silica column (eluent: mixture of methylene chloride
20 and of ethyl acetate) and recrystallization from isopropanol, 500 mg (1.26 mmol) of the expected product are obtained.
Melting point: 159-160°C
111 NMR (d6-DMSO) : 8 (ppm) : 1.04 (t, 3H), 1.21 (t, 3H), 25 3.28 (q, 2H), 3.55 (q, 2H), 7.45 (m, 3H), 7.7 (m, 5H), 7.9 (dxd, 1H).
 
19
Example 3 (Compounds Nos 3 and 4)
7-Chloro-2-(pyrid-4-y1)-4,5-dihydro-214-benzo[e]indazol¬1-y1 N,N-diethylcarbamate hydrochloride (Compound
No. 3) and 7-chloro-3-(pyrid-4-y1)-4,5-dihydro-31I-
5 benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride (Compound No. 4)
3.1 Methyl 6-chloro-2-hydroxy-3,4-dihydro-1-
naphthoate
10.1 g (252 mmol) of 60% sodium hydride in
10 oil, 621 ml of toluene and 14.18 ml (163 mmol) of dimethyl carbonate are introduced into a 2 1 reactor. The reaction mixture is stirred at reflux for 1 h. A solution of 15.2 g (84 mmol) of 6-chloro-3,4-dihydro-11I-naphthalen-2-one in 268 ml of toluene is
15 subsequently added. The reaction mixture is heated at reflux for 24 h. The reaction mixture is subsequently cooled to 0°C and then acidified by addition of 92 ml of acetic acid. 114 ml of water are added and the
separated organic phase is separated by settling and
20 washed with three times 150 ml of water and then with 100 ml of a saturated aqueous sodium chloride solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced pressure. The residue is purified by chromatography on a silica
25 column (elution being carried out with a mixture of cyclohexane and of dichloromethane) to result in 12.8 g (53.6 mmol) of the expected product used as is in the
 
20
following stage.
3.2 7-Chloro-l-hydroxy-4,5-dihydro-2H¬benzo[e]indazole
28 g (117 mmol) of the product obtained in
5 Stage 3.1 and 28.6 ml (586.6 mmol) of hydrazine
monohydrate are introduced into a 2 1 reactor. The mixture is dissolved in 782 ml of acetic acid and heated at reflux for 4 h. The reaction mixture is subsequently cooled and then concentrated under reduced
10 pressure. The resulting product is taken up in 300 ml of ethyl acetate and 300 ml of water. The organic phase is separated by settling and washed twice with 200 ml of water and then once with 200 ml of a saturated aqueous sodium chloride solution. The organic phase is
15 subsequently dried over magnesium sulphate and then concentrated under reduced pressure. The residue obtained is triturated from 200 ml of ethyl ether and then filtered off to result in 25 g (113.3 mol) of the expected product.
20 Melting point: 232-233°C
3.3 7-Chloro-1-hydroxy-2-(pyrid-4-y1)-4,5- dihydro-2H-benzo[e]indazole and 7-chloro-l-hydroxy-3- (pyrid-4-y1)-4,5-dihydro-3H-benzo[e]indazole
15.2 g (68.8 mmol) of the product obtained in
25 Stage 3.2, 16.95 g (82.66 mmol) of 4-iodopyridine, 4.14 ml (34.44 mmol) of trans-1,2-diaminocyclohexane, 1.31 g (6.89 mmol) of copper iodide and 36.55 g
 
21
(172.2 mmol) of potassium phosphate are introduced under an inert atmosphere into a 2 1 reactor. The reaction mixture is suspended in 690 ml of dioxane, brought to reflux for 24 h and then cooled. The mixture
5 is concentrated under reduced pressure and then taken up in 200 ml of water. This aqueous phase is acidified
to pH 5 by successive additions of acetic acid. The suspension is stirred for 30 minutes and then the precipitate obtained is filtered off, washed with water
10 and then dried under reduced pressure to produce 16.6 g (55.7 mmol) of the expected N-arylation product in the form of a mixture of isomers.
LC-MS: 2 peaks at 60.4% and 38% corresponding to [MH] = 298.
15    3.4 7-Chloro-2-(pyrid-4-y1)-4,5-dihydro-2H-
benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride (Compound No. 3) and 7-chloro-3-(pyrid-4-y1)-4,5- dihydro-3H-benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride (Compound No. 4)
20    13 g (43.66 mmol) of the mixture of isomers
obtained in Stage 3.3, 18.1 g (131 mmol) of finely ground potassium carbonate and 11.07 ml (87.32 mmol) of N,N-diethylcarbamoyl chloride are introduced, under an
inert atmosphere, into a 2 1 reactor. The reaction
25 mixture is suspended in 1 1 of acetonitrile, brought to reflux for 24 h and then cooled. The reaction mixture is concentrated under reduced pressure. The resulting
 
22
product is taken up in 300 ml of ethyl acetate and 300 ml of water. The organic phase is separated by
settling and washed twice with 200 ml of water and once with 200 ml of a saturated aqueous sodium chloride
5 solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced pressure. The resulting product comprises the two positional isomers (Compounds Nos 3 and 4). The latter are separated by column chromatography (300 g of Merck
10 15-40 microns silica, eluents: mixture of heptane and of ethyl acetate).
7-Chloro-2-(pyrid-4-y1)-4,5-dihydro-2H-benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride (Compound No. 3)
15    Product No. 3, thus isolated, is converted to
the hydrochloride by dissolving in a 0.1N solution of hydrochloric acid in isopropanol. The solution is concentrated to dryness under reduced pressure. After triturating from ethyl ether, filtering and drying
20 under reduced pressure, 2.3 g (5.79 mmol) of the final product are obtained.
Melting point: 250-251°C
1H NMR (d6-DMS0) : 8 (ppm) : 1.08 (t, 3H), 1.31 (t, 3H), 2.98 (m, 4H), 3.3 (q, 2H), 3.68 (q, 3H), 7.27 (d, 1H),
25 7.38 (dxd, 1H), 7.46 (dxd, 1H), 7.92 (d, 2H), 8.87 (d, 2H).
7 -Chloro -3 - (pyrid-4 -y1) -4,5 - dihydro-3 H-
 
23
benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride (Compound No. 4)
Product No. 4, thus isolated, is converted to the hydrochloride by dissolving in a 0.1N solution of
5 hydrochloric acid in isopropanol. The solution is concentrated to dryness under reduced pressure. After
triturating from ethyl ether, filtering and drying under reduced pressure, 6.2 g (15.62 mmol) of the final product are obtained.
10 Melting point: 224-226°C
1H NMR (d6-DMS0): 8 (ppm) : 1.18 (t, 3H) , 1.29 (t, 3H), 3.02 (t, 2H), 3.31 (m, 4H), 3.51 (q, 2H), 7.20 (d, 1H),
7.32 (dxd, 1H), 7.45 (d, 1H), 8.08 (d, 2H), 8.98 (d, 2H).
15
Example 4 (Compound No. 5) 8-Methoxy-3-(pyrid-4-y1)-4,5-dihydro-3H¬benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride
4.1 Methyl 6-methoxy-2-hydroxy-3,4-dihydro-1-
20 naphthoate
3.4 g (85.12 mmol) of 60% sodium hydride in oil, 180 ml of toluene and 4.78 ml (56.75 mmol) of dimethyl carbonate are introduced into a 1 1 reactor. The reaction mixture is stirred at reflux for 1 h. A
25 solution of 5 g (28.37 mmol) of 7-methoxy-3,4-dihydro¬1H-naphthalen-2-one in 100 ml of toluene is
subsequently added. The reaction mixture is heated at
 
24
reflux for 24 h. The reaction mixture is subsequently cooled to 0°C and then acidified by addition of 30 ml of acetic acid. 30 ml of water are added and the separated organic phase is separated by settling and
5 washed with two times 50 ml of water and then with
50 ml of a saturated aqueous sodium chloride solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced pressure. After purification by chromatography on a silica column
10 (eluent: mixture of methylene chloride and of heptane), 3.9 g (16.64 mmol) of the expected product are obtained and are used as in the following stage.
4.2 1-Hydroxy-8-methoxy-4,5-dihydro-214- benzo[e]indazole
15    3.9 g (16.65 mmol) of the product obtained in
Stage 4.1 and 4.06 ml (83.24 mmol) of hydrazine monohydrate are introduced into a 0.5 1 reactor. The mixture is dissolved in 166 ml of acetic acid and heated at reflux for 4 h. The reaction mixture is
20 subsequently cooled and then concentrated under reduced pressure. The resulting product is taken up in 100 ml of ethyl acetate and 100 ml of water. The organic phase is separated by settling and washed twice with 100 ml of water and then once with 100 ml of a saturated
25 aqueous sodium chloride solution. The organic phase is
subsequently dried over magnesium sulphate and then concentrated under reduced pressure. The residue
 
25
obtained is triturated from 50 ml of ethyl ether and then filtered off to result in 2.4 g (11.1 mmol) of the expected product.
4.3 1-Hydroxy-8-methoxy-3-(pyrid-4-y1)-4,5- 5 dihydro-3H-benzo [e] indazole
1.2 g (5.55 mmol) of the product obtained in Stage 4.2, 1.36 g (6.66 mmol) of 4-iodopyridine,
0.33 ml (2.77 mmol) of trans-1,2-diaminocyclohexane, 0.105 g (0.55 mmol) of copper iodide and 2.94 g
10 (13.87 mmol) of potassium phosphate are introduced, under an inert atmosphere, into a 0.1 1 reactor. The reaction mixture is suspended in 55 ml of dioxane, brought to reflux for 24 h and then cooled. The mixture
is subsequently taken up in 1 1 of a 1/1 mixture of
15 water and of ethyl acetate. The organic phase is separated by settling and then washed with water
(50 ml). This aqueous phase is acidified to pH 5 by successive additions of acetic acid. The precipitate obtained is filtered off, washed with water and then
20 dried under reduced pressure to produce 0.2 g
(0.68 mmol) of the expected N-arylation product. The organic phase is dried over magnesium sulphate and then concentrated under reduced pressure. The resulting product is purified by chromatography on a silica
25 column (eluents: mixture of dichloromethane and methanol) to produce an additional 0.57 g (1.94 mmol) of the expected N-arylation product.
 
26
1H NMR (d6-DMSO) : 8 (ppm) : 2.89 (dxd, 2H), 3.09 (dxd, 2H), 3.73 (s, 3H), 6.2 (dxd, 1H), 7.1 (m, 2H), 7.48 (m, 2H), 8.6 (m, 2H).
4.4 8-Methoxy-3-(pyrid-4-y1)-4,5-dihydro-3H¬5 benzo[e]indazol-1-y1 N,N-diethylcarbamate hydrochloride (Compound No. 5)
0.77 g (2.63 mmol) of the product obtained in Stage 4.3, 1.09 g (7.88 mmol) of finely ground
potassium carbonate and 0.67 ml (5.25 mmol) of
10 N,N-diethylcarbamoyl chloride are introduced, under an inert atmosphere, into a 0.1 1 reactor. The reaction mixture is suspended in 30 ml of acetonitrile, brought to reflux for 24 h and then cooled. The reaction
mixture is concentrated under reduced pressure. The
15 resulting product is taken up in 100 ml of ethyl acetate and 100 ml of water. The organic phase is separated by settling and washed twice with 50 ml of water and then once with 50 ml of a saturated aqueous sodium chloride solution. The organic phase is
20 subsequently dried over magnesium sulphate and then concentrated under reduced pressure. The resulting product is purified by column chromatography (90 g of Merck 15-40 microns silica, eluents: mixture of heptane and of ethyl acetate). The compound thus isolated is
25 recrystallized from isopropanol and then redissolved in
a 0.1N solution of hydrochloric acid in isopropanol.
The solution is concentrated to dryness under reduced
 
27
pressure. After triturating from ethyl ether, filtering off and drying under reduced pressure, 234 mg
(0.59 mmol) of the expected compound are obtained.
Melting point: 225-227°C
5    1H NMR    (d6-DMSO) : 8    (ppm) :    1.15    (t,    3H) ,    1.3    (t,    3H),
2.92    (t,    2H),    3.2-3.4    (m,    4H),    3.51    (q,    2H),    3.71    (s,
3H) ,    6.8    (m,    2H) ,    7.2    (d,    1H),    7.98    (d,    2H) ,    8.82    (d,
2H).                           

10 Example 5 (Compound No. 82) 7-Chloro-4,5-dihydro-3-(pyrid-4-y1)-3H-benzo[e]indazol¬1-y1 N,N-diisopropylcarbamate hydrochloride
2.3 g (7.72 mmol) of the mixture of isomers obtained in Stage 3.3 of Example 3, in solution in
15 30 ml of dimethylformamide, are added dropwise, under an inert atmosphere and at 0°C, to a suspension of 0.4 g (10.04 mmol) of sodium hydride in 17 ml of dimethylformamide in a 1 1 reactor. After stirring at
ambient temperature for 1 hour, 1.39 g (8.5 mmol) of
20 N,N-diisopropylcarbamoyl chloride in 30 ml of dimethylformamide are added dropwise. The reaction mixture is stirred at ambient temperature for 18 h and is then concentrated under reduced pressure. The resulting product is taken up in 300 ml of ethyl
25 acetate and 300 ml of water. The aqueous phase is brought to pH 5 by addition of acetic acid. The organic phase is separated by settling and washed twice with
 
28
200 ml of water and then once with 200 ml of a saturated aqueous sodium chloride solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced pressure. The resulting
5 product is purified by column chromatography (300 g of Merck 15-40 microns silica, eluents: mixture of dichloromethane and of ethyl acetate). The product thus isolated is converted to the hydrochloride by dissolution in a 0.1N solution of hydrochloric acid in
10 isopropanol. The solution is concentrated to dryness under reduced pressure. After triturating from ethyl ether, filtering off and drying under reduced pressure,
1.2 g (2.6 mmol) of the final product are obtained.
15    Melting point: 237-269°C
1H NMR    (d6-DMS0) :    8    (ppm):    1.29    (m,    12H),    3.02    (m,    2H),
3.19    (m,    2H) ,    3.91    (m,    1H),    4.2    (m,    1H),    7.2    (d,    1H),
7.35    (dxd,    IH),    7.42    (s,    1H),    7.6    (d,    2H),    8.7    (d,    2H).

Example 6 (Compound No. 84)
20 7-Chloro-3-(pyrid-4-y1)-3H-benzo[e]indazol-1-y1 N,N¬dii sopropylcarbamate hydrochloride
1.65 g (3.38 mmol) of 7-chloro-4,5-dihydro-3- (pyrid-4-y1)-3H-benzo[e]indazol-1-y1 N,N-diisopropyl¬carbamate, prepared according to the method described
25 in Example 5, 1.105 g (6.21 mmol) of N-bromosuccinimide and 0.127 g (0.78 mmol) of 2,2'-azobis(2- methylpropionitrile) are added to a 100 ml reactor. The
 
29
mixture, dissolved in 40 ml of carbon tetrachloride, is
stirred at reflux for 24 h and is then concentrated under reduced pressure. The resulting product is taken up in 300 ml of dichloromethane and 2 ml of a
5 concentrated aqueous ammonia solution. The organic phase is separated and washed with 200 ml of water and then with 200 ml of a saturated aqueous sodium chloride solution. The organic phase is subsequently dried over magnesium sulphate and then concentrated under reduced
10 pressure. The resulting product is purified by chromatography on an alumina column (eluents: mixture of dichloromethane and of methanol) and then on a silica column (eluents: mixture of dichloromethane and of ethyl acetate). The product thus isolated is
15 converted to the hydrochloride by dissolution in a 0.1N solution of hydrochloric acid in isopropanol. The solution is concentrated to dryness under reduced pressure. After triturating from ethyl ether, filtering off and drying under reduced pressure, 1.2 g (2.6 mmol)
20 of the final product are obtained.
Melting point: 234-248°C
11.1 NMR (d6-DMSO) : 8 (ppm) : 1.42 (m, 12H) , 4.02 (m, 1H), 4.41 (m, 1H), 7.6 (dxd, 1H), 7.8 (m, 3H), 7.92 (d, 2H), 8.15 (d, 1H), 8.79 (d, 2H).
25    The chemical structures and the physical
properties of a few compounds of general formula (I) according to the invention are illustrated in the
 
30
following Table 2.
In the "Salt" column of this table, "HC1" denotes a hydrochloride and "-" denotes a compound in the base state. The base:acid molar ratios are shown
5 opposite. The "M.p." column gives the melting points of the products, the amorphous compounds being characterized by their mass spectrometry (MS)
analytical results.
10
Table 2
 

 
     N
R2


No.    Xi    X2    X3        W    N Ri R2     C4•C5 bond    Y    Salt    M.p. (°C)
141-
                                       
1    H    F    H    H    0    N(CH2CH3)2    single    2-(4-fluorophenyl)    -    142
2    H    F    H    H    0    N(CH2CH3)2    double    2-(4-fluorophenyl)    -    159-160
                                    HCI    25
3    H    CI    H    H    0    N(CH2CH3)2    single    2-(pyrid-4-yl)    1:1    251
4    H    CI    H    H    0    N(CH2CH3)2    single    3-(pyrid-4-yl)    HCI    224-
 
31
No.    X1    X2        X4        W    NR., R2    C4-05 bond    Y    Salt    M.p. (°C)
                                    1:1    226
                                    HCI    225-
5    H    H    OCH3    H        0    N (CH2C H3)2    single    3-(pyrid-4-y1)    1:1    227
                                        130 -
6    H    H    H    H    0    N(CH3)2    single    2-(4-methylphenyl)    -    131
7    H    H    H    H    0    N(CH2CH3)2    single    2-(4-chlorophenyl)    -    148-149
                        /— \                150-
8    H    H    H    H    0    —►\2    single    2-(3-chlorophenyl)    -    151
9    H    H    H    H    0    N(CH3)2    single    2-(3-chlorophenyl)    -    128- 129
10    H    H    H    H    0    N(CH2CH3)2    single    2-(3-chlorophenyl)    -    111-
113
11    H    H    H    H    0    —NO    single    2-(3-chlorophenyl)    -    1155;
157
12    H    H    H    H    0    N(CH(CH3)2)2    single    2-(3-chlorophenyl)    -    116- 118
13    H    H    H    H    0    N(CH2CH3)2    single    2-phenyl    -    124- 125
14    H    CI    H    H    0    N(CH2CH3)2    single    2-phenyl    -    114- 116
15    H    H    H    H    0    N(CH3)Ph    single    2-(3-chlorophenyl)    -    75-
78
16    H    H    H    H    0    N(CH2CH3)2    single    2-(2-chlorophenyl)    -    396*
17    H    H    H    H    0    N(CH2CH3)2    single    2-ethyl    -    6 601 -
18    H    H    H    H    0    N(CH3)Ph    single    2-ethyl    -    348*
19    H    H        H    0    N(CH3)Ph    single    2-(2,2,2 -
trifluoroethyl)    -    402*
20    H    H    H    H    0    N(CH2CH3)2    single    2-(4-fluorophenyl)    -    113323-
21    H    H    H    H    0    N(CH(CH3)2)2    single    2-(4-fluorophenyl)    -    995-6
22    H    H    H    H    0    N(CH3)Ph    single    2-(4-fluorophenyl)    -    119;-
                                        211-
23    H    H    H    H    0    NO    single    2-(4-fluorophenyl)    -    212
24    H    H    H    H    0    0    single    2-(4-fluorophenyl)    -    117745-
                        /—\                175-
25    H    H    H    H    0    r,k_ Jo    single    2-(4-fluorophenyl)    -    176
                                        211-
26    H    H    H    H    0    N(CH3)2    single    2-(4-chlorophenyl)    -    212
                                        203-
27    H    H    H    H    0    N(CH(C H3)2)2    single    2-(4-chlorophenyl)    -    204
                                        202-
28    H    H    H    H    0    N(CH3)Ph    single    2-(4-chlorophenyl)    -    203
29    H    H    H    H    0    NO    single    2-(4-chlorophenyl)    -    2112
213
                                        200-
30    H    H    H    H    0    0    single    2-(4-chlorophenyl)    -    201
 
32
No.    X1    X2    X3    X4        W    NRIR2    C4•C5 bond    Y    Salt    M.p.
(°C)
                        rTh                185-
31        H    H    H    0    I.Lio    single    2-(4-chlorophenyl)    -    186
32    H    H    H    H    0    /—\
- Me
I \ 2    single    2-(4-chlorophenyl)    -    157 -
158
33    H    H    H    H    0    N(CH3)2    single    2-(4-fluorophenyl)    -    216- 217
                        /----\                167-
34    H    H    H    H    0    nk ju-me    single    2-(4-fluorophenyl)    -    168
35    H    H    H    H    0    N(CH3)2    single    2-phenyl    -    158- 159
36    H    H    H    H    0    N(CH(CH3)2)2    single    2-phenyl    -    146- 147
                                        169-
37    H    H    H    H    0    N(CH3)Ph    single    2-phenyl    -    170
                                        176-
38    H    H    H    H    0    N    sing le    2-phenyl    -    177
                                        155-
39    H    CI    H    H    0    N (CH2C H3)2    sing le    2-(4-fluorophenyl)    -    156
40    H    H    H    H    0    N(CH3)- (CH2)2CH3    single    2-(4-fluorophenyl)    -    143-
144
                                2-(3-chloro-4-        99-
41    H    H    H    H    0    N(CH2CH3)2    single    methylphenff    -    101
42    H    H    H    H    0    N(CH2CH3)2    single    2-(3-chloro-4- fluorophenyl)    -    148-
150
43    H    Cl    H    H    0    N(CH2CH3)2    single    2-(3-chloro-4-
methylphenyl)    -    131-
133
                                2-(2,4-        78-
44    H    Cl    H    H    0    N(CH2CH3)2    single    difluorophenyl)    -    80
                                    HCI    298-
45    H    H    H    H    0    N(CH2CH3)2    single    2-(pyrid-4-y1)    1 :1    299
46    H    CI    H    H    0    N(CH2CH3)2    sing le    2-(pyrid-2-yl)    -    110 108-
47    H    OCH3    H    H    0    N(CH2CH3)2    single    2-(4-fluorophenyl)    -    116612-
                                        162-
48    H    Br    H    H    0    N(CH2CH3)2    single    2-(4-fluorophenyl)    -    163
                                2-(3-chloro-4-        108-
49    H    CI    H    H    0    N(CH2CH3)2    single    fluorophenyl)    -    110
50    H    CI    H    H    0    N(CH2CH3)2    single    2-methyl    -    117- 118
51    H    H    H    H    0    N(CH2CH3)2    double    2-(3-chlorophenyl)    -    112256-
52    H    H    H    H    0    N(CH2CH3)2    double    2-(4-fluorophenyl)    -    1125-
126
53    H    H    H    H    0    N(CH3)(C-    1-102double    2-(4-fluorophenyl)    -    115545-
                        Ctii               
54    H    H    H    H    0    N(CH2CH3)2    double    2-(3-chloro-4-
fluorophenyl)    -    412*
                                2-(3-chloro-4-        118-
55    H    H    H    H    0    N(CH2CH3)2    double    methylphenyl)    -    120
56    H    CI    H    H    0    N(CH2CH3)2    double    2-(4-fluorophenyl)    -    151- 153
                                2-(3-chloro-4-        141-
57    H    Cl        H    0    N(CH2CH3)2    double    fluorophenyft    -    143
58    H    CI    H    H    0    N(CH2CH3)2    double    2-(3-chloro-4-    -    141-
 
33
No.    XI    X2    X3    Xs        W    NR,R2    bond    C4 -.p.
Y    Salt    M
(°C)
                                methylphenyl)        143
59    H    CI    H    H    0    N(CH2CH3)2    double    2,42,4-
difluorophenyl)    -    144-
146
60    H    OCH3    H    H    0    N(CH2CH3)2    single    3"(PYrid"411)    HCI
1:1 :1    208-
220
61    H    CH3    H    H    0    N(CH2CH3)2    single    3-(pyrid-4-yl)    HCI
1:1    222-
225
                                    HCI    194 -
62    H    CI    H    H    0    N(CH2CH3)2    single    3-(pyrid-3-yl)    1 :1    197
63    H    CI    H    H    0    N(CH2CH3)2    single    2-(pyrid-3-yl)    HCI
1 :1    - 1557
15
64    H    F    H    H    0    N(CH2CH3)2    single    2-(pyrid-4-y1)    HCI
1 :1    324-
326
65    H    F    H    H    0    N(CH2CH3)2    single    3-(pyrid-4-y1)    HCI
1 :1    233-
242
                                        131 -
66    H    CI    H    H    0    N(CH2CH3)2    single    3-(4-fluorophenyl)    -    132
67    H    CI    H    H    0    N(CH2CH3)2    single    3-(pyrid-2-y1)    HCI
1 :1    122-
•    123
                                        128-
68    H    CI    H    H    0    N(CH2CH3)2    single    2-(3-fluorophenyl)    -    130
69    H    CI    H    H    0    N(CH2CH3)2    single    3-(3-fluorophenyl)    -    124- 126
                                    HCI    300 -
70    H    Br    H    H    0    N(CH2CH3)2    single    2-(pyrid-4-y1)    1 :1    310
71    H    Br    H    H    0    N(CH2CH3)2    single    3-(pyrid-4-y1)    HCI
1 :1    233 -
238
72    H    CI    H    H    0    N(CH2CH3)2    single    3-(4-methylphenyl)    -    115559"
73    H    CI    H    H    0    N(CH2CH3)2    single    2-(4-methylphenyl)    -    111281-
74    H    CI    H    H    0    N(CH2CH3)2    single    2-(2-fluorophenyl)        414*
75    H    CI    H    H    0    N(CH2CH3)2    single    3-(4-chlorophenyl)    -    177
178
76    H    CI    H    H    0    N(CH2CH3)2    single    2-(4-chlorophenyl)    -    148 -
149
77    H    Cl    H    H    0    N(CH2CH3)2    single    2 -(4-
methoxypheny I    -    131 -
132
                                3-(4-        185 -
78    H    Cl    H    H    0    N(CH2CH3)2    single    methoxyphenyl)    "    187
79    H    CI    H    H    0    N(CH2CH3)2    double    3-(3-fluorophenyl)    -    114424"
                                       
SO    H    CI    H    H    0    N(CH3)Ph    single    3-(pyrid-4-yl)    -    213 -
215
                                        184 -
81    H    CI    H    H    0    N(CH(CH3)2)2    single    2-(pyrid-4-yl)    "    185
                                        146
                                    -    —
82    H    CI    H    H    0    N(CH(CH3)2)2    single    3-(pyrid-4-yl)        148
                                    HCI    237 -
                                    1 :1    269
                                    HCI    238 -
83    OCH3        H    H    H    0    N(CH2CH3)2    single    3-(pyrid-4-yl)    1:1    240
84    H    CI    H    H    0    N(CH(CH3)2)2    double    3-(pyrid-4-y1)    HCI
1 :1    234
8
248
 
34
No.    X,    X2    X3    X4    W    NR, R2    C4-05 bond    Y    -Salt    M.p.
(°C)
85    H    Cl    H    H    0        single    2-(pyrid-411)    HCI
1:1    153 -
173
86    H    Cl    H    H    0        single    3_    HCI HCI 1 :1    243 -
248
87    H    CI    H    H    0    N(CH(CH3)2)2    single    2-(pyrid-3-y1)    HCI
1 :1    243
26-
0
88    H    CI    H    H    0    N(CH(CH3)2)2    single    3-(pyrid-3-y1)    HCI
1 :1    209 -
213
89    H    CI    H    H    S    N(CH(CH3)2)2    single    34pyricm_yi)    HCI
1 .1I    2    -
318
8
90    H    CI    H    H    0        single    3-(pyrid-4-y1)    HCI
1 :1    228 -
252
91    H    CI    H    H    0    NCH3C(CH43    single    2-(pyrid-4-y1)    H1 911    33560 -
0
92    H    CI    H    H    0    N(CH(CH3)2)2    double    3-(pyrid-3-y1)    HCI
1 :1    238 -
240
93    H    CI    H    H    0    N(CH(CH3)2)2    single    3-(pyrid-2-y1)    -    193 -
195
94    H    F    H    H    0    N(CH(CH3)2)2    single    3-(pyrid-4-y1)    HCI
1    :1    255 -
260
95    H    Cl    H    H    0    N(CH(CH3)2)2    single    3-(PYrid-2-y1)    -    222
96    H    CI    H    H        N(CH(CH3)2)2    single    2-(pyrazin-2-yl)    -    175-
176
97    H    F    H    H    0    N(CH(CH3)2)2    double    3-(pyrid-4-yl)    HCI
1 :1    272
0
280
98    H    CI    H    H    O    N(CH(CH3)2)2    double    3-(pyrid-2-y1)    -    213 -
215
99    H    Cl    H    H    ,...,
"""    N(CI-13) - (C(CH3)3)    single    3-(pyrid-4-yl)    HCI
1:1    411*
100    H    CI    H    H    0    N(CH2CH3)2    double    3-(pyrid-4-y1)    HCI
1 :1    227 -
229
101    H    CI    H    H    0    N(CH3)Ph    double    3-(pyrid-4-y1)    HCI
1 :1    248 -
268
*[MH]
The compounds of the invention have been subjected to pharmacological tests which have demonstrated their advantage as substances with
5 therapeutic activities.
The compounds of the invention also exhibit characteristics of solubility in water which promote good in vivo activity.
 
35
Study of the binding of [3H]Ro5-4864 to peripheral-type  benzodiazepine receptors (PBR or p sites)
The affinity of the compounds of the invention for PBR or p sites (sites of binding of
5 peripheral type to benzodiazepines) was determined.
The p site receptors can be labelled selectively in rat kidney membranes incubated in the presence of [3H]Ro5-4864. The compounds of the invention have formed the subject of an in vitro study with
10 respect to their affinity for these receptors.
The animals used are male Sprague-Dawley rats (Iffa Credo) weighing 180 to 300 g. After decapitation, the kidney is removed and the tissue is homogenized at 4°C using a PolytronTM homogenizer for 2 min at 6/10 of
15 the maximum speed in 35 volumes of 50 mM Na2HPO4 phosphate buffer at a pH adjusted to 7.5 with NaH2PO4. The membrane homogenate is filtered through gauze and diluted tenfold with buffer.
[3H]Ro5-4864 (specific activity:
20 70-90 Ci/mmol; New England Nuclear), at a concentration of 0.5 nM, is incubated in the presence of 100 pl of the membrane homogenate in a final volume of 1 ml of buffer comprising the test compound.
After incubating for 3 h at 0°C, the
25 membranes are recovered by filtration through Whatman GF/BTM filters washed with 2 times 4.5 ml of cold (0°C)
 
36
incubation buffer. The amount of radioactivity retained by the filter is measured by liquid scintigraphy.
For each concentration of studied compound, the percentage of inhibition of the binding of
5 [3H]Ro5-4864 is determined and then the IC50 concentration, the concentration which inhibits 50% of the specific binding, is determined.
The IC50 values of the most active compounds of the invention range from 0.5 nM to 300 nM. In
10 particular, compounds Nos 14, 20 and 56 in Table 2
exhibit respective IC50 values of 1.6 nM, 2.8 nM and 1.4 nM.
The compounds of the invention are therefore ligands with an affinity for peripheral-type
15 benzodiazepine receptors.
Study of the neuroprotective activity
Test of survival of the motor neurons after sectioning the facial nerve in rats aged 4 days
20    After lesion of the facial nerve in immature
rats, the motor neurons of the facial nucleus
experience neuronal death by apoptosis. Neuronal survival is evaluated using neuronal counting and histological methods.
25    Immature rats aged 4 days are anaesthetized
with pentobarbital (3 mg/kg by the i.p. route). The right facial nerve is exposed and sectioned at its
 
37
outlet from the stylomastoid foramen. After waking up, the young rats are returned to their mothers and are treated for 7 days with one or two daily
administrations, by the oral or intraperitoneal route, 5 at doses ranging from 1 to 10 mg/kg.
7 days after the lesion, the animals are decapitated and the brains are frozen in isopentane at -40°C. The entire facial nerve is cut with a cryostat into sections with a width of 10 Am. The motor neurons
10 are stained with cresyl violet and counted using the HistoTM software (BiocomTM) .
In this model, the compounds of the invention increase neuronal survival by 38 to 78%. The results of the test of survival of the motor neurons for compounds
15 Nos 14, 20 and 56 of the table are presented in Table 3 below.
Table 3
No.    14    20    56
% increase in the survival
of the neurons
(10 mg/kg pc)    38%    59%    74%

The results of the tests show that the most 20 active compounds of the invention promote neuroprotection.
The compounds of the invention can thus be used for the preparation of medicaments, in particular
 
38
for the preparation of a medicament intended to prevent or treat a pathology in which peripheral-type benzodiazepine receptors are involved.
Thus, according to another of its aspects, a subject-matter of the invention is medicaments which comprise a compound of formula (I), or an addition salt of the latter with a pharmaceutically acceptable acid, or a hydrate or a solvate.
These medicaments are employed in
10 therapeutics, in particular in the prevention and/or treatment of various types of peripheral neuropathies, such as traumatic or ischaemic neuropathies,
infectious, alcoholic, diabetic, medicinal or genetic neuropathies, and motor neuron conditions, such as
15 spinal amyotrophies and amyotrophic lateral sclerosis. These medicaments will also find an application in the treatment of neurodegenerative diseases of the central nervous system, either of acute type, such as strokes and cranial and medullar traumas, or of chronic type,
20 such as autoimmune diseases (multiple sclerosis), Alzheimer's disease, Parkinson's disease and any other disease in which the administration of
neuroprotective/neurotrophic factors is supposed to have a therapeutic effect.
25    The compounds of the invention can also be
used for the preparation of medicaments intended for the prevention and/or treatment of anxiety, of epilepsy
 
39
and of sleep disorders. This is because ligands of the PBR or p sites stimulate the production of neurosteroids, such as pregnenolone, dehydroepiandro¬sterone and 3a-hydroxy-5a-pregnan-20-one, by promoting
5 the transfer of cholesterol from the outside to the inside of the mitochondrial membrane. These neurosteroids modulate the activity of the GABAA-chloride channel macromolecular complex and can thus produce anxiolytic, anticonvulsant and sedative
10 activities.
The compounds of the invention can also be used in the treatment of acute or chronic renal insufficiency, of glomerulonephritis, of diabetic nephropathy, of cardiac ischaemia and cardiac
15 insufficiency, of myocardial infarction, of ischaemia of the lower limbs, of coronary vasospasm, of angina pectoris, of pathologies associated with the heart valves, of inflammatory heart diseases, of side effects due to cardiotoxic medicaments or as a result of heart
20 surgery, of atherosclerosis and of its thromboembolic complications, of restenosis, of graft rejections, or
of conditions related to incorrect proliferation or incorrect migration of smooth muscle cells.
Furthermore, recent data in the literature
25 indicate that the peripheral-type benzodiazepine
receptor might play a fundamental role in the
regulation of cell proliferation and cancerization
 
40
processes. Generally, and in comparison with normal tissues, an increased density of peripheral-type benzodiazepine receptors is observed in various types of tumours and cancers.
5    In human astrocytomas, the level of
expression of the peripheral-type benzodiazepine receptor is correlated with the degree of malignancy of the tumour, the proliferation index and the survival of the patients. In human cerebral tumours, the increase
10 in the number of peripheral-type benzodiazepine receptors is used as a diagnostic indication in medical imaging and as a therapeutic target for conjugates formed from a ligand of the peripheral-type benzodiazepine receptor and from a cytostatic drug. A
15 high density of peripheral-type benzodiazepine receptors is also observed in ovarian carcinomas and breast cancers. As regards the latter, it has been demonstrated that the level of expression of the peripheral-type benzodiazepine receptors is related to
20 the aggressive potential of the tumour; furthermore,
the presence of a peripheral-type benzodiazepine
receptor agonist stimulates the growth of a mammary cancer line.
These combined results, which suggest a
25 deleterious function of the peripheral-type
benzodiazepine receptor in cancerization processes, constitute a relevant basis for the search for
 
41
synthetic ligands specific for the peripheral-type benzodiazepine receptor which are capable of blocking the effects thereof.
The compounds can therefore be used for the 5 treatment of tumours and cancers.
The peripheral-type benzodiazepine receptors are also present in the skin and, in this respect, the compounds which can be used according to the invention can be used for the prophylaxis or the treatment of
10 cutaneous stress.
The term "cutaneous stress" is understood to mean the various situations which might cause damage,
in particular to the epidermis, whatever the agent which causes this stress. This agent can be internal
15 and/or external to the body, such as a chemical or free-radical agent, or else external, such as ultraviolet radiation.
Thus, the compounds which can be used according to the invention are intended to prevent and
20 to combat cutaneous irritation, dry patches, erythemas, dysaesthetic sensations, heating sensations, pruritus of the skin and/or mucous membranes, or ageing, and can also be used in cutaneous disorders, such as, for example, psoriasis, pruriginous diseases, herpes,
25 photodermatoses, atopic dermatitides, contact dermatitides, lichens, prurigo, pruritus, insect
stings, in fibroses and other disorders of collagen
 
42
maturation, in immunological disorders or in dermatological conditions, such as eczema.
The compounds of the invention can also be used for the prevention and treatment of chronic
5 inflammatory diseases, in particular rheumatoid arthritis, and pulmonary inflammatory diseases.
According to another of its aspects, the present invention relates to pharmaceutical
compositions comprising, as active principle, a
10 compound according to the invention. These pharmaceutical compositions comprise an effective dose of at least one compound according to the invention, or
a pharmaceutically acceptable salt, a hydrate or a solvate of the said compound, and at least one
15 pharmaceutically acceptable excipient. The said excipients are chosen, according to the pharmaceutical form and the method of administration desired, from the usual excipients known to a person skilled in the art.
In the pharmaceutical compositions of the
20 present invention for oral, sublingual, subcutaneous, intramuscular, intravenous, topical, local, intratracheal, intranasal, transdermal or rectal
administration, the active principle of formula (I) above, or its optional salt, solvate or hydrate, can be
25 administered in unit administration form, as a mixture with conventional pharmaceutical excipients, to animals and human beings for the prophylaxis or treatment of
 
43
the above disorders or diseases.
The appropriate unit administration forms comprise oral forms, such as tablets, soft or hard gelatin capsules, powders, granules and oral solutions
5 or suspensions, sublingual, buccal, intratracheal, intraocular and intranasal administration forms, forms for administration by inhalation, topical, transdermal, subcutaneous, intramuscular or intravenous administration forms, rectal administration forms and
10 implants. The compounds according to the invention can be used, for topical application, in creams, gels, ointments or lotions.
By way of example, a unit administration form of a compound according to the invention in the tablet 15 form can comprise the following components:
Compound according to the invention    50.0 mg
Mannitol    223.75 mg
Croscarmellose sodium    6.0 mg
Maize starch    15.0 mg
20 Hydroxypropylmethylcellulose    2.25 mg
Magnesium stearate    3.0 mg
The said unit forms comprise doses in order to make possible daily administration of 0.001 to 20 mg of active principle per kg of body weight, depending on
25 the pharmaceutical dosage form.
There may be specific cases where higher or lower dosages are appropriate; such dosages do not
depart from the scope of the invention. According to
 
44
the usual practice, the dosage appropriate to each patient is determined by the physician according to the method of administration and the weight and response of the said patient.
5    The present invention, according to another
of its aspects, also relates to a method for the
treatment of the pathologies indicated above which comprises the administration, to a patient, of an effective dose of a compound according to the
10 invention, or one of its pharmaceutically acceptable salts or hydrates or solvates.
 
CLAIMS
1.    Compound corresponding to the
formula (I)
 
5 in which
W represents an oxygen or sulphur atom;
X1, X2, X3 and X4 each represent, independently of one another, a hydrogen or halogen atom or a cyano, Cl-C6-alkyl, Cl-C6-fluoroalkyl, C1-05-alkoxy or
10 C1-C6-fluoroalkoxy group;
Y is in the (N2) or (N3) position;
when Y is in the (N2) position, Y represents a C1-C6-alkyl, Cl-C6-fluoroalkyl, aryl or heteroaryl group; when Y is in the (N3) position, Y represents an aryl or
15 heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from halogen atoms or Cl-C6-alkyl, C1-C6-alkoxy, Cl-C6-alkylthio, Cl-C6-alkyl-S (0) -, C1-C6-alkyl-S(0)2- or
20 Cl-C6-fluoroalkyl groups;
the bond in the C4-05 position is a double or single bond;
 
46
R1 and R2 each represent, independently of one another, an aryl, benzyl or C1-C6-alkyl group; or else R1 and R2 form, with the nitrogen atom which carries them, a heterocycle optionally substituted by one or more C1-C6-
5 alkyl or benzyl groups;
in the form of the base or of an addition salt with an acid, and in the hydrate or solvate form.
2.    Compound of formula (I) according to
Claim 1, characterized in that
10 W represents an oxygen or sulphur atom;
X1, X2 and X3 each represent, independently of one another, a hydrogen atom, a halogen atom, a cyano group, a C1-C6-alkyl group or a C1-C6-alkoxy group; X4 represents a hydrogen atom;
15 Y is in the (N2) or (N3) position;
when Y is in the (N2) position, Y represents a
C1-C6-alkyl group, a C1-C6-fluoroalkyl group, an aryl group or a heteroaryl group;
when Y is in the (N3) position, Y represents an aryl 20 group or a heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from halogen atoms, C1-C6-alkyl groups and C1-C6-alkoxy groups;
25 the bond in the C4-05 position is a double or single bond;
R1 and R2 each represent, independently of one another,
 
47
an aryl group or a C1-C6-alkyl group; or else R1 and R2
form, with the nitrogen atom which carries them, a heterocycle optionally substituted by one or two C1-C6-alkyl groups;
5 in the form of the base or of an addition salt with an acid, and in the hydrate or solvate form.
3.    Compound of formula (I) according to
Claim 1 or 2, characterized in that
W represents an oxygen or sulphur atom;
10 X1, X2 and X3 each represent, independently of one another, a hydrogen atom, a halogen atom, a C1-C6-alkyl group or a Cl-C6-alkoxy group;
X4 represents a hydrogen atom;
Y is in the (N2) or (N3) position and represents an 15 aryl group or a heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from halogen atoms, C1-C6-alkyl groups and C1-C6-alkoxy groups;
20 the bond in the C4-05 position is a double or single bond;
R1 and R2 each represent, independently of one another, an aryl group or a Cl-C6-alkyl group; or else R1 and R2 form, with the nitrogen atom which carries them, a
25 heterocycle optionally substituted by one or two C1-C6¬alkyl groups;
in the form of the base or of an addition salt with an
 
48
acid, and in the hydrate or solvate form.
4.    Compound of formula (I) according to any one of Claims 1 to 3, characterized in that
W represents an oxygen or sulphur atom;
5 X1, X2 and X3 each represent, independently of one another, a hydrogen atom, a halogen atom, a C1-C6-alkyl group or a Cl-C6-alkoxy group;
X4 represents a hydrogen atom;
Y is in the (N3) position and represents an aryl group 10 or a heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from halogen atoms, C1-C6-alkyl groups and C1-C6-alkoxy groups;
15 the bond in the C4-05 position is a double or single bond;
R1 and R2 each represent, independently of one another, an aryl group or a C1-C6-alkyl group; or else Ri and R2 form, with the nitrogen atom which carries them, a
20 heterocycle optionally substituted by one or two C1-C6¬alkyl groups;
in the form of the base or of an addition salt with an acid, and in the hydrate or solvate form.
5.    Process for the preparation of a
25 compound of formula (I) according to any one of Claims 1 to 4, characterized in that
a mixture composed of the positional isomers of general
 
49
formulae (Va) and (Vb),
 
in which X1, X2, X3, X4 and Y are as defined in the
5 general formula (I) according to Claim 1 and in which the Y group is respectively in the (N2) position and in the (N3) position of the pyrazole ring,
is reacted with a carbamoyl chloride derivative of general formula C1C(W)NR1R2, in which W, R1 and R2 are as
10 defined in the general formula (I) according to Claim 1, in the presence of a base,
to obtain, after separation, the compounds of general formulae (Ia) and (Ib),
 

 
the single bond in the C4-05 position subsequently being optionally dehydrogenated to form a double bond. 6.    Process for the preparation of a
 
compound of formula (1a)
 

 
in which
5 W represents an oxygen or sulphur atom;
X1, X2, X3 and X4 each represent, independently of one another, a hydrogen or halogen atom or a cyano, Cl-C6-alkyl, Cl-C6-fluoroalkyl, Cl-C6-alkoxy or C1-C6-fluoroalkoxy group;
10 Y is in the (N2) position and represents a C1-C6-alkyl, C1-C6-fluoroalkyl, aryl or heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from halogen atoms or Cl-C6-alkyl, Cl-C6-alkoxy,
15 Cl-C6-alkylthio, 01-C6-alkyl-9(0)-, C1-C6-alkyl-S(0)2- or C1-C6-fluoroalkyl groups;
the bond in the C4-05 position is a double or single bond;
R1 and R2 each represent, independently of one another, 20 an aryl, benzyl or C1-C6-alkyl group; or else R1 and R2
form, with the nitrogen atom which carries them, a
 
51
heterocycle optionally substituted by a C1-C6-alkyl or benzyl group;
characterized in that the compound of general formula (Va),
 

 
5
in which X1, X2, X3, X4 and Y are as defined in the
general formula (Ia) above,
is reacted with a carbamoyl chloride derivative of general formula C1C(W)NR1R2, in which W, R1 and R2 are as
10 defined in the general formula (Ia) above, in the presence of a base,
the single bond in the C4-05 position subsequently being optionally dehydrogenated to form a double bond. 7.    Compound of formula (Va)
Xl
x, OOP    (Va)
15    X4 HO NY
in which
X1, X2, X3 and X4 each represent, independently of one another, a hydrogen or halogen atom or a cyano, Cl-C6-alkyl, C1-C6-fluoroalkyl, Cl-C6-alkoxy or
 
52
Cl-C6-fluoroalkoxy group;
Y represents a Cl-C6-alkyl, Cl-C6-fluoroalkyl, aryl or heteroaryl group;
the aryl or heteroaryl groups optionally being
5 substituted by one or more atoms or groups chosen from halogen atoms or C1-C6-alkyl, Cl-C6-alkoxy, Cl-C6-
alkylthio, Cl-C6-alkyl-S(0)-, C1-C6-alkyl-S(0)2- or C1-C6-fluoroalkyl groups.
8.    Compound of formula (Vb)
10
 

 
HO
in which
Xi, X2, X3 and X4 each represent, independently of one another, a hydrogen or halogen atom or a cyano,
15 C1-C6-alkyl, Cl-C6-fluoroalkyl, Cl-C6-alkoxy or C1-C6-fluoroalkoxy group;
Y represents an aryl or heteroaryl group;
the aryl or heteroaryl groups optionally being substituted by one or more atoms or groups chosen from
20 halogen atoms or Cl-C6-alkyl, Cl-C6-alkoxy, Cl-C6- alkylthio, Cl-C6-alkyl-S(0)-, C1-C6-alkyl-S(0)2- or Cl-C6-fluoroalkyl groups.
9.    Medicament, characterized in that it
 
53
comprises a compound of formula (I) according to any
one of Claims 1 to 4, or an addition salt of this compound with a pharmaceutically acceptable acid, or a
hydrate or a solvate of the compound of formula (I).
5    10. Pharmaceutical composition,
characterized in that it comprises a compound of formula (I) according to any one of Claims 1 to 4, or a pharmaceutically acceptable salt, a hydrate or a solvate of this compound, and at least one
10 pharmaceutically acceptable excipient.
11.    Use of a compound of formula (I) according to any one of Claims 1 to 4 for the preparation of a medicament intended to prevent or treat a pathology in which peripheral-type
15 benzodiazepine receptors are involved.
12.    Use of a compound of formula (I) according to Claim 11 for the preparation of a medicament intended to prevent or treat peripheral neuropathies, motor neuron conditions,
20 neurodegenerative diseases of the central nervous
system, anxiety, epilepsy, sleep disorders, acute or chronic renal insufficiency, glomerulonephritis,
diabetic nephropathy, cardiac ischaemia and cardiac insufficiency, myocardial infarction, ischaemia of the
25 lower limbs, coronary vasospasm, angina pectoris, pathologies associated with the heart valves, inflammatory heart diseases, side effects due to
 
54
cardiotoxic medicaments or as a result of heart surgery, atherosclerosis and its thromboembolic complications, restenosis, graft rejections, conditions related to incorrect proliferation or incorrect
5 migration of smooth muscle cells, tumours and cancers, cutaneous stress, chronic inflammatory diseases, in particular rheumatoid arthritis, and pulmonary inflammatory diseases.
 
54
ca:diotoxio medtcaments or as a z:a5ult of heart surgery, aLherosolerosis and its thromboembolie complicationSp r4*Lanosis, graft rejections, conditions related to incorrect proliferation or Incorrect
5 migration of smooch xuscLe cells, tAmaars and Cancers.
cataneous stress. Chronic Inflammatory d_i_eases. Jo particular rheumatoid azthritia, and palmunary Inflammatory &senses,
 
indexation.Ist QCOK tags.Ist

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